CLEC4E monoclonal antibody, clone AT16E3
产品名称： CLEC4E monoclonal antibody, clone AT16E3
英文名称： CLEC4E monoclonal antibody, clone AT16E3
产品编号： MAB8479
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against partial recombinant CLEC4E.
- Immunogen:
- Recombinant protein corresponding to amino acids 41-219 of human CLEC4E.
- Host:
- Mouse
- Reactivity:
- Human
- Form:
- Liquid
- Isotype:
- IgG2b, kappa
- Storage Buffer:
- In PBS, pH 7.4 (0.1% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:250)
Western Blot (1:250-1:500)
Immunofluorescence (1:250-1:500)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Mincle is a long sought receptor for mycobacterial cord factor.
Matsunaga I, Moody DB.J Exp Med. 2009 Dec 21;206(13):2865-8. Epub 2009 Dec 14.
- 2.
- Direct recognition of the mycobacterial glycolipid, trehalose dimycolate, by C-type lectin Mincle.
Ishikawa E, Ishikawa T, Morita YS, Toyonaga K, Yamada H, Takeuchi O, Kinoshita T, Akira S, Yoshikai Y, Yamasaki S.J Exp Med. 2009 Dec 21;206(13):2879-88. Epub 2009 Dec 14.
- 3.
- The Dectin-2 family of C-type lectins in immunity and homeostasis.
Graham LM, Brown GD.Cytokine. 2009 Oct-Nov;48(1-2):148-55. Epub 2009 Aug 7.
- Applications
- Western Blot (Cell lysate)
- Cell lysates of HeLa (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with CLEC4E monoclonal antibody, clone AT16E3 (Cat # MAB8479) (1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Immunohistochemistry paraffin embedded sections of human colorectal cancer tissue were incubated with monoclonal CLEC4E monoclonal antibody, clone AT16E3 (Cat # MAB8479) (1 : 50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
- Entrez GeneID:
- 26253
- Protein Accession#:
- NP_055173
- Gene Name:
- CLEC4E
- Gene Alias:
- CLECSF9,MINCLE
- Gene Description:
- C-type lectin domain family 4, member E
- Omim ID:
- 609962
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein is a downstream target of CCAAT/enhancer binding protein (C/EBP), beta (CEBPB) and may play a role in inflammation. Alternative splice variants have been described but their full-length sequence has not been determined. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq
- Other Designations:
- C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 9,macrophage-inducible C-type lectin